منابع مشابه
Electronic fingerprinting of RNA.
Software has been developed to assist RNA fingerprinting analysis. One program generates, from a DNA sequence data file, the oligonucleotides resulting from digestion of an RNA transcript labeled with any specified nucleotide(s). Oligonucleotides are sorted according to their position on the fingerprint. Expected molar yields and products of secondary redigestion are also indicated. A second pr...
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چکیده ندارد.
15 صفحه اولRNA fingerprinting by molecular indexing.
Class IIS restriction enzymes, a subgroup of class II, cleave DNA at a precise location outside their recognition sites, and produce overhangs of unknown sequences (1). Molecular indexing is a series of techniques designed to characterize DNA fragments by these unknown sequences (2–4). I applied this principle for description of the total mRNA population using a 3′ end cDNA fragment generated b...
متن کاملPhysico-chemical fingerprinting of RNA genes
We advance here a novel concept for characterizing different classes of RNA genes on the basis of physico-chemical properties of DNA sequences. As knowledge-based approaches could yield unsatisfactory outcomes due to limitations of training on available experimental data sets, alternative approaches that utilize properties intrinsic to DNA are needed to supplement training based methods and to ...
متن کاملApproaches to sequence analysis of 125I-labeled RNA
A method is described for the initial steps of sequence analysis of RNase T1-and pancreatic RN-ase-resistant oligonucleotides of RNA containing cytidylate residues labeled in vitro with 125I. In many cases an oligonucleotide sequence can be deduced from a consideration of (i) its relative position in the two-dimensional fingerprint (with DEAE thin layer homochromatographic second dimension), (i...
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ژورنال
عنوان ژورنال: Nature
سال: 1973
ISSN: 0028-0836,1476-4687
DOI: 10.1038/246483a0